Composite

Part:BBa_K3610055:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-09)


FKBP / LargeBit NanoLuc for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 541
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Short linker between FKBP and LargeBit NanoLuc that interferes as little as possible with the two sequences. Additionally, the codon optimized version of the split-NanoLuc protein was taken.

Source

K3610022, K416001, BBa_K3610014

References

Inobe, Tomonao; Nukina, Nobuyuki (2016): Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins. In: Journal of bioscience and bioengineering 122 (1), S. 40–46. DOI: 10.1016/j.jbiosc.2015.12.004.

Ding, Lin; Laor, Dana; Weisman, Ronit; Forsburg, Susan L. (2014): Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast. In: Yeast (Chichester, England) 31 (7), S. 253–264. DOI: 10.1002/yea.3014.